The source for the inbred line B73 used to make the BAC libraries that were sequenced is available from the North Central Regional Plant Introduction Station through the U.S. National Plant Germplasm System under the accession PI 550473. When requesting seed from the North Central Regional Plant Introduction Station, ask for any lot descended from the Coe PI 550473 lines.
Sequencing and Assembly Information
Sequencing technologies: Sanger
Assembly methods: phredPhrap v. 2009 and ABySS v. 1.2.7 Construction of pseudomolecules: Map-based order and orientation of a BAC tiling path.
n/a (mostly re-ordering/addition of contigs/fosmids)
A contig is a contiguous consensus sequence that is
derived from a collection of overlapping reads.
A scaffold is set of a ordered and orientated contigs
that are linked to one another by mate pairs of sequencing reads.